Anticorps Monoclonal anti-Beclin 1

Beclin 1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C10C4

N° de cat : 66665-1-PBS

Synonymes

BECN1, 1C10C4, ATG 6, ATG6, Beclin1

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Galerie de données de validation

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  WB analysis using 66665-1-Ig (same clone as 66665-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66665-1-Ig (Beclin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66665-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 66665-1-Ig (same clone as 66665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66665-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 66665-1-Ig (same clone as 66665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66665-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66665-1-Ig (same clone as 66665-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66665-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66665-1-Ig (same clone as 66665-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66665-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51448-1

  Cytometric bead array sample test of MP51448-1, Beclin 1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66665-1-PBS. Detection antibody: 66665-2-PBS.

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  Cytometric bead array standard curve of MP51448-1

  Cytometric bead array standard curve of MP51448-1, Beclin 1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66665-1-PBS. Detection antibody: 66665-2-PBS. Standard:Ag1843. Range: 1.563-200 ng/mL.

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Informations sur le produit

66665-1-PBS cible Beclin 1 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Beclin 1 Protéine recombinante Ag1843
• Nom complet: beclin 1, autophagy related
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52-60 kDa
• Numéro d’acquisition GenBank: BC010276
• Symbole du gène: Beclin 1
• Identification du gène (NCBI): 8678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.

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