Anticorps Monoclonal anti-Beta-2-Microglobulin

Beta-2-Microglobulin Monoclonal Antibody for IF/ICC, FC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF/ICC, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1H3F4

N° de cat : 68395-1-PBS

Synonymes

B2M, 1H3F4, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3

---

Galerie de données de validation

• 

  IF Staining of NCCIT using 68395-1-Ig (same clone as 68395-1-PBS)

  Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 68395-1-Ig (same clone as 68395-1-PBS)

  Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 68395-1-Ig (same clone as 68395-1-PBS)

  1x2*10^6 Jurkat cells were surface stained with 1 μg Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig, Clone:1H3F4) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(N/A), or 1 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were not fixed. This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68395-1-PBS cible Beta-2-Microglobulin dans les applications de IF/ICC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Beta-2-Microglobulin Protéine recombinante Eg31815
• Nom complet: beta-2-microglobulin
• Masse moléculaire calculée: 119 aa, 14 kDa
• Poids moléculaire observé: 12-14 kDa
• Numéro d’acquisition GenBank: BC032589
• Symbole du gène: B2M
• Identification du gène (NCBI): 567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.

{{ptg:RelatedPrimaryAntibodies}}