Anticorps Recombinant de lapin anti-Beta Actin
Beta Actin Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, porc, rat, souris
Applications
WB, IHC, IF/ICC, IP, Indirect ELISA
Conjugaison
Non conjugué
CloneNo.
4H1
N° de cat : 81115-1-PBS
Synonymes
Galerie de données de validation
Informations sur le produit
81115-1-PBS cible Beta Actin dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris
Réactivité | Humain, porc, rat, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Recombinant |
Type | Anticorps |
Immunogène | Beta Actin Protéine recombinante Ag14521 |
Nom complet | actin, beta |
Masse moléculaire calculée | 375 aa, 42 kDa |
Poids moléculaire observé | 42 kDa |
Numéro d’acquisition GenBank | BC002409 |
Symbole du gène | Beta Actin |
Identification du gène (NCBI) | 60 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS only |
Conditions de stockage | Store at -80°C. 20ul contiennent 0,1% de BSA. |
Informations générales
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )