Anticorps Polyclonal de lapin anti-Beta Actin

• Phare
• Validé par KD/KO

Beta Actin Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, singe, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

N° de cat : CL647-20536

Synonymes

Actin, ACTB, bate actin, β actin, Beta-actin

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Galerie de données de validation

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  IF Staining of HepG2 using CL647-20536

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 Beta Actin antibody (CL647-20536) at dilution of 1:200.

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  FC experiment of HepG2 using CL647-20536

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Beta Actin (CL647-20536) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-20536 cible Beta Actin dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons canin, Humain, rat, singe, souris

• Réactivité: canin, Humain, rat, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Beta Actin Protéine recombinante Ag14521
• Nom complet: actin, beta
• Masse moléculaire calculée: 375 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC002409
• Symbole du gène: Beta Actin
• Identification du gène (NCBI): 60
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

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