Anticorps Monoclonal anti-Beta Actin

Beta Actin Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, plante, poisson-zèbre, rat, souris et plus (5)

Applications

WB, IF, ELISA

Conjugaison

HRP

Publications

315

CloneNo.

7D2C10

Avis (9)

N° de cat : HRP-60008

Synonymes

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP β actin, HRP-ACTB, HRP-Beta actin, HRP-F actin, PS1TP5BP1, β actin

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Galerie de données de validation

• 

  WB analysis of multi-cells/tissue using HRP-60008

  Western blot analysis of beta actin in various tissues and cell lines using Proteintech antibody HRP-60008 at a dilution of 1:5000.

• 

  WB analysis of HeLa using HRP-60008

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60008 (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 304 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

HRP-60008 cible Beta Actin dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, plante, poisson-zèbre, rat, souris

• Réactivité: Humain, plante, poisson-zèbre, rat, souris
• Réactivité citée: rat, canin, Humain, porc, poulet, singe, souris, Hamster
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: actin, beta
• Masse moléculaire calculée: 375 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC002409
• Symbole du gène: ACTB
• Identification du gène (NCBI): 60
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publications

Species	Application	Title
	WB	Cell; Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate; Authors - Kazushige Shiraishi
	WB	Nat Neurosci; Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.; Authors - Kornélia Szebényi
mouse	WB	Blood; Fetal liver hepcidin secures iron stores in utero.; Authors - Lara Kämmerer
human	WB	Nat Microbiol; Adeno-associated virus 2 bound to its cellular receptor AAVR.; Authors - Ran Zhang
human	WB	Mol Cancer; CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.; Authors - Ya Fan
	WB	Acta Pharm Sin B; Targeting a cryptic allosteric site of SIRT6 with small-molecule inhibitors that inhibit the migration of pancreatic cancer cells.; Authors - Qiufen Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maria (Verified Customer) (10-24-2023)

Used Incubation over night at 4°C, never tested a shorter incubation, but it worked very nice.

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: whole lysate in UREA made from muscles, brown fat and white fat. species: mice and naked molerats

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Wojciech (Verified Customer) (04-20-2023)

The beta actin works very well diluted at 1:5000. It was used on T-ALL cell lines. Loading 30 ug of whole protein lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: T cells

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 5000
• Cell Tissue Type: Caco2, Mice colon

FH

Iru (Verified Customer) (06-07-2021)

The antibody works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using HRP- conjugated beta actin in NSC34 cell line at dilution of 1:10000

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: NSC34 cells

HRP Beta Actin Antibody Western Blot validation (1:10000 dilution) in NSC34 cells (Cat no:HRP-60008)

FH

Hasibur (Verified Customer) (08-01-2019)

Nice antibody and no need for secondary antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: Bladder Cancer

FH

Joanne (Verified Customer) (01-22-2019)

Tested using 10ug of Caco-2 cell lysate and whole membrane. A strong band with no background and a great time saver!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: caco-2 cell

HRP Beta Actin Antibody Western Blot, validation (1:5000 dilution) in caco-2 cell (Cat no:HRP-60008)

FH

Tess (Verified Customer) (11-29-2018)

Little to no background, and takes half the time.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: human colorectal cancer cell line

HRP Beta Actin Antibody Western Blot, validation (1:10,000 dilution) in human colorectal cancer cell line (Cat no:HRP-60008)

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