Anticorps Monoclonal anti-Beta Arrestin 2

Beta Arrestin 2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E8F2

N° de cat : 68502-1-Ig

Synonymes

ARRB2, β-Arrestin 2, BARR2, Arrestin beta-2, ARR2

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Galerie de données de validation

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  WB analysis using 68502-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 68502-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 68502-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 68502-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 68502-1-Ig

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 68502-1-Ig

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68502-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Beta Arrestin 2 antibody (68502-1-Ig, Clone: 1E8F2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68502-1-Ig cible Beta Arrestin 2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Beta Arrestin 2 Protéine recombinante Ag29807
• Nom complet: arrestin, beta 2
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC007427
• Symbole du gène: Beta Arrestin 2
• Identification du gène (NCBI): 409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

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