Anticorps Recombinant de lapin anti-Beta Catenin

Beta Catenin Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

2J24

N° de cat : 80488-1-PBS

Synonymes

2J24, b cat, B-catenin, beta-Catenin, Catenin beta 1

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Galerie de données de validation

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  WB analysis using 80488-1-RR (same clone as 80488-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  WB analysis of zebrafish using 80488-1-RR (same clone as 80488-1-PBS)

  zebrafish tissue was subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 80488-1-RR (same clone as 80488-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (80488-1-RR, Clone: 2J24 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HCT 116 using 80488-1-RR (same clone as 80488-1-PBS)

  Chromatin was prepared from HCT 116 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of β-Catenin (80488-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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Informations sur le produit

80488-1-PBS cible Beta Catenin dans les applications de WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, poisson-zèbre, porc, rat, souris

• Réactivité: Humain, poisson-zèbre, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: catenin (cadherin-associated protein), beta 1, 88kDa
• Masse moléculaire calculée: 781 aa, 86 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC058926
• Symbole du gène: Beta Catenin
• Identification du gène (NCBI): 1499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

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