Anticorps Polyclonal de lapin anti-Beta TRCP

Beta TRCP Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28393-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28393-1-AP (same clone as 28393-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28393-1-AP (Beta TRCP antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28393-1-AP (BETA-TRCP,BTRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28393-1-AP (BETA-TRCP,BTRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28393-1-PBS cible Beta TRCP dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Beta TRCP Protéine recombinante Ag28739
• Nom complet: beta-transducin repeat containing
• Masse moléculaire calculée: 605 aa, 69 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC027994
• Symbole du gène: Beta TRCP
• Identification du gène (NCBI): 8945
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BTRC, also named as BTRCP, FBW1A, and FBXW1A, is an F-box family protein. It is a Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. BTRC targets many important proteins with diverse functions, such as p53, HRAS, NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation. It is required for the activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB, and TNF. Required for proteolytic processing of GLI3.

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