Anticorps Monoclonal anti-Beta Galactosidase

• Phare
• Validé par KD/KO

Beta Galactosidase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

4F4F4

N° de cat : 66586-1-Ig

Synonymes

GLB1, 4F4F4, Acid beta-galactosidase, Beta-galactosidase, EBP

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Galerie de données de validation

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  WB analysis using 66586-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A549 using 66586-1-Ig

  WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells.

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  IHC staining of human kidney using 66586-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66586-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66586-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Beta Galactosidase antibody (66586-1-Ig, Clone: 4F4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

66586-1-Ig cible Beta Galactosidase dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Beta Galactosidase Protéine recombinante Ag8069
• Nom complet: galactosidase, beta 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 64-66 kDa, 76-85 kDa
• Numéro d’acquisition GenBank: BC007493
• Symbole du gène: GLB1/Beta-galactosidase
• Identification du gène (NCBI): 2720
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.

Publications

Species	Application	Title
sheep	IF	Antioxidants (Basel); Effects of Fisetin Treatment on Cellular Senescence of Various Tissues and Organs of Old Sheep; Authors - Charles A Huard
rabbit	IHC	Am J Sports Med; Effects of Losartan and Fisetin on Microfracture-Mediated Cartilage Repair of Ankle Cartilage in a Rabbit Model; Authors - Ingrid K Stake
mouse	WB,IHC	Free Radic Biol Med; MnSOD non-acetylation mimic knock-in mice exhibit dilated cardiomyopathy; Authors - Joseph R Schell
human	WB,IF	Noncoding RNA Res; LncRNA NEAT1-206 regulates autophagy of human umbilical cord mesenchymal stem cells through the WNT5A/Ca2+ signaling pathway under senescence stress; Authors - Weili Wang
rat	WB	Life Sci; Alda-1 mediates cell senescence and counteracts bone loss in weightlessness through regulating mitophagy; Authors - Jinpeng Wang
	IF	J Gerontol A Biol Sci Med Sci; Ferroptosis activation contributes to kidney aging in mice by promoting tubular cell senescence; Authors - Zheng Wang

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