Anticorps Monoclonal anti-Biglycan

Biglycan Monoclonal Antibody for Indirect ELISA, IHC, WB

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

Indirect ELISA, IHC, WB

Conjugaison

Non conjugué

CloneNo.

1E3F2

N° de cat : 67275-1-PBS

Synonymes

BGN, 1E3F2, PG-S1, PGI, PG S1

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Galerie de données de validation

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  WB analysis of pig skin using 67275-1-Ig (same clone as 67275-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 67275-1-Ig (same clone as 67275-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67275-1-Ig (same clone as 67275-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 67275-1-Ig (same clone as 67275-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67275-1-Ig (same clone as 67275-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Biglycan Monoclonal antibody (67275-1-Ig, Clone:1E3F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67275-1-PBS cible Biglycan dans les applications de Indirect ELISA, IHC, WB et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Biglycan Protéine recombinante Ag8012
• Nom complet: biglycan
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC004244
• Symbole du gène: Biglycan
• Identification du gène (NCBI): 633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

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