Anticorps Recombinant de lapin anti-Biotin

Biotin Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

n/a

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242355B1

N° de cat : 98598-1-RR

Synonymes

Vitamin B7, Vitamin H

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Galerie de données de validation

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  FC experiment of human PBMCs using 98598-1-RR

  1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98598-1-RR

  1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15) and then stained with 0.25 ug Anti-Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98598-1-RR

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98598-1-RR

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4) and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines, splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98598-1-RR cible Biotin dans les applications de FC et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Biotin-KLH
• Nom complet: Biotin
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Biotin, also called vitamin H or vitamin B7, is a water-soluble vitamin required by all organisms (PMID: 12055344). It serves as a necessary cofactor for enzymes involved in carboxylation reactions essential for gluconeogenesis, lipid metabolism, and amino acid catabolism (PMID: 38825069). Biotin is often used to label proteins and nucleic acids. This anti-biotin antibody can be used for detecting biotinylated molecules.

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