Anticorps Monoclonal anti-BrdU

BrdU Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B10E12

N° de cat : 66241-1-PBS

Synonymes

5-bromo-2-deoxyuridine, BrdUrd, Bromodeoxyuridine, BUdR

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Galerie de données de validation

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  IHC staining of mouse spleen using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

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  IF Staining of L-929 using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed L-929 cells using BrdU antibody (66241-1-Ig, Clone: 1B10E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 66241-1-Ig (BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using 66241-1-Ig(BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66241-1-PBS cible BrdU dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Small molecule
• Nom complet: BrdU
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf

Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf

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