Anticorps Polyclonal de lapin anti-Brevican

Brevican Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19017-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human brain using 19017-1-AP (same clone as 19017-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 19017-1-AP (same clone as 19017-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 19017-1-AP (same clone as 19017-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 19017-1-AP (same clone as 19017-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19017-1-AP (same clone as 19017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19017-1-AP (Brevican antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19017-1-PBS cible Brevican dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Brevican Protéine recombinante Ag13552
• Nom complet: brevican
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 145 kDa, 72kDa, 80 kDa
• Numéro d’acquisition GenBank: BC010571
• Symbole du gène: BCAN
• Identification du gène (NCBI): 63827
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

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