Anticorps Recombinant de lapin anti-CD117/c-Kit

CD117/c-Kit Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

241313C5

N° de cat : APC-98377

Synonymes

CD117, KIT, C Kit, c-Kit, EC:2.7.10.1

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Galerie de données de validation

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  FC experiment of TF-1 using APC-98377

  1x10^6 TF-1 cells were surface stained with 5 ul APC Anti-Human C-Kit Rabbit RecAb (APC-98377, Clone: 241313C5) (red) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9) (blue). Cells were not fixed.

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  FC experiment of human blood cells using APC-98377

  1x10^6 human blood cells were surface stained with FITC Plus Anti-Human CD34, and 5 ul APC Anti-Human C-Kit Rabbit RecAb (APC-98377, Clone: 241313C5) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: human blood cells, cellules TF-1

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-98377 cible CD117/c-Kit dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Masse moléculaire calculée: 976 aa, 110 kDa
• Numéro d’acquisition GenBank: BC071593
• Symbole du gène: CD117
• Identification du gène (NCBI): 3815
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

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