Anticorps Polyclonal de lapin anti-APC15

APC15 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 20409-1-AP

Synonymes

C11orf51, HSPC020, Anaphase-promoting complex subunit 15, ANAPC15

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Galerie de données de validation

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  WB analysis of HT-29 using 20409-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20409-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse adrenal gland using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat adrenal gland using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HEK-293
• Résultats positifs en IHC: tissu placentaire humain, tissu de glande surrénale de rat, tissu pancréatique humain, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu pulmonaire humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20409-1-AP cible APC15 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APC15 Protéine recombinante Ag14237
• Nom complet: chromosome 11 open reading frame 51
• Masse moléculaire calculée: 121 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC005156
• Symbole du gène: APC15
• Identification du gène (NCBI): 25906
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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