Anticorps Recombinant de lapin anti-C11orf68

C11orf68 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242221C11

N° de cat : 84725-5-PBS

Synonymes

242221C11, BLES03, P5326, Protein p5326, UPF0696 protein C11orf68

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Galerie de données de validation

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  WB analysis of A431 using 84725-5-RR (same clone as 84725-5-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 84725-5-RR (C11orf68 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 84725-5-RR (same clone as 84725-5-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 84725-5-RR (C11orf68 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84725-5-RR (same clone as 84725-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84725-5-RR (C11orf68 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84725-5-RR (same clone as 84725-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C11orf68 antibody (84725-5-RR, Clone: 242221C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84725-5-RR (same clone as 84725-5-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug C11orf68 Recombinant antibody (84725-5-RR, Clone:242221C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84725-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84725-5-RR against Human C11orf68 were performed. The affinity constant is 11.8 nM.

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Informations sur le produit

84725-5-PBS cible C11orf68 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: C11orf68 Protéine recombinante Ag36023
• Nom complet: chromosome 11 open reading frame 68
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC010512
• Symbole du gène: C11orf68
• Identification du gène (NCBI): 83638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BLES03 (basophilic leukemia-expressed protein 03) is a human protein of unknown function. Recent studies have shown that BLES03 is a novel eukaryotic phosphothreonine cleavage enzyme involved in the biosynthesis of dehydrogenated amino acids.The crystal structure of BLES03 has been resolved by single-wavelength anomalous scattering (SAD) with a resolution of 2.5 Å.

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