Anticorps Polyclonal de lapin anti-Hikeshi

• Phare
• Validé par KD/KO

Hikeshi Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 14808-1-AP

Synonymes

C11orf73, HSPC138, HSPC179, HSPC248, L7RN6

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Galerie de données de validation

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  WB analysis of mouse kidney using 14808-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 14808-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14808-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14808-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14808-1-AP

  IP result of anti-Hikeshi (IP:14808-1-AP, 4ug; Detection:14808-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human kidney using 14808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14808-1-AP (Hikeshi antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14808-1-AP (Hikeshi antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 14808-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14808-1-AP (Hikeshi antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 14808-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14808-1-AP (Hikeshi antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A375, cellules PC-3, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

14808-1-AP cible Hikeshi dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Hikeshi Protéine recombinante Ag6530
• Nom complet: chromosome 11 open reading frame 73
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC001677
• Symbole du gène: Hikeshi
• Identification du gène (NCBI): 51501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
		Oncogene; HSP110 promotes colorectal cancer growth through STAT3 activation.; Authors - K Berthenet
human	WB	J Med Genet; Leukoencephalopathy and early death associated with an Ashkenazi-Jewish founder mutation in the Hikeshi gene.; Authors - Simon Edvardson
human	WB	Int J Mol Med; HIKESHI silencing can enhance mild hyperthermia sensitivity in human oral squamous cell carcinoma HSC‑3 cells.; Authors - Yoshiaki Tabuchi ; KD Validated
mouse,human	WB,IHC	Pharmaceutics; Role of HIKESHI on Hyperthermia for Castration-Resistant Prostate Cancer and Application of a Novel Magnetic Nanoparticle with Carbon Nanohorn for Magnetic Hyperthermia; Authors - Takashi Nagai ; KD Validated
mouse	WB	Front Genet; Nuclear Accumulation of HSP70 in Mouse Skeletal Muscles in Response to Heat Stress, Aging, and Unloading With or Without Reloading.; Authors - Antonios Apostolopoulos

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