Anticorps Polyclonal de lapin anti-SDH5

SDH5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 19906-1-AP

Synonymes

C11orf79, hSDH5, PGL2, SDH assembly factor 2, SDH5, SDHAF2, SDHAF2, PGL2, SDH5

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Galerie de données de validation

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  WB analysis using 19906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19906-1-AP (SDH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SDH5 antibody (19906-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

19906-1-AP cible SDH5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SDH5 Protéine recombinante Ag13893
• Nom complet: chromosome 11 open reading frame 79
• Masse moléculaire calculée: 166 aa, 20 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC002331
• Symbole du gène: SDH5
• Identification du gène (NCBI): 54949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SDH5, also named asC11orf79, SDHAF2 and SDHAF2, is a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. SDH5 has been shown to be mutated in patients with paragangliomas, also known as familial non-chromaffin paragangliomas type 2. Catalog#19906-1-AP is a rabbit polyclonal antibody raised against the full-length of human SDH5.

Publications

Species	Application	Title
human	WB	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma
human	WB	Apoptosis; GAS6/Axl is associated with AMPK activation and attenuates H2O2-induced oxidative stress; Authors - Zhenxing Liang
mouse	WB	Cell Biochem Funct; Pterostilbene protects against H2 O2 -induced oxidative stress by regulating GAS6/Axl signaling in HL-1 cells; Authors - Wencheng Di
mouse	WB,IHC,IF	Biochim Biophys Acta Mol Basis Dis; Succinate dehydrogenase mediated ROS production contributes to ASIC1a-induced chondrocyte pyroptosis in rheumatoid arthritis; Authors - Wenqiang Liu

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