Anticorps Recombinant de lapin anti-ASUN

ASUN Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241586E9

N° de cat : 84252-4-PBS

Synonymes

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

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Galerie de données de validation

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  WB analysis using 84252-4-RR (same clone as 84252-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84252-4-RR (same clone as 84252-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01142-1

  Cytometric bead array standard curve of MP01142-1, ASUN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84252-4-PBS. Detection antibody: 84252-3-PBS. Standard: Ag13727. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84252-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84252-4-RR against Human ASUN were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84252-4-PBS cible ASUN dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ASUN Protéine recombinante Ag13727
• Nom complet: chromosome 12 open reading frame 11
• Masse moléculaire calculée: 706 aa, 80 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC003081
• Symbole du gène: ASUN
• Identification du gène (NCBI): 55726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).

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