Anticorps Polyclonal de lapin anti-C12orf29

• Phare
• Validé par KD/KO

C12orf29 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31647-1-PBS

Synonymes

RNA Ligase, RNA Ligase 1, Rnl

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Galerie de données de validation

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  WB analysis using 31647-1-AP (same clone as 31647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31647-1-AP (C12orf29 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 31647-1-AP (same clone as 31647-1-PBS)

  WB result of C12orf29 antibody (31647-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C12orf29 transfected HeLa cells. This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 31647-1-AP (same clone as 31647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31647-1-AP (C12orf29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 31647-1-AP (same clone as 31647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31647-1-AP (C12orf29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 31647-1-AP (same clone as 31647-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C12orf29 antibody (31647-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31647-1-PBS cible C12orf29 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C12orf29 Protéine recombinante Ag35681
• Nom complet: chromosome 12 open reading frame 29
• Masse moléculaire calculée: 37kDa，325aa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: NM_001009894.2
• Symbole du gène: C12orf29
• Identification du gène (NCBI): 91298
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).

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