Anticorps Polyclonal de lapin anti-VIPAS39

VIPAS39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 20771-1-AP

Synonymes

C14orf133, hSPE 39, Protein spe 39 homolog, VIPAR, VIPAS39, VPS33B interacting protein

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Galerie de données de validation

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  WB analysis using 20771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 20771-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 20771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20771-1-AP (VIPAS39 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20771-1-AP (VIPAS39 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20771-1-AP

  Immunofluorescent analysis of Hela cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

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  IF Staining of HepG2 using 20771-1-AP

  Immunofluorescent analysis of HepG2 cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20771-1-AP

  Immunofluorescent analysis of Hela cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HEK-293 using 20771-1-AP

  Immunofluorescent analysis of 293 cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules L02, tissu cérébral de souris, tissu rénal humain
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HEK-293, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

20771-1-AP cible VIPAS39 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VIPAS39 Protéine recombinante Ag14762
• Nom complet: chromosome 14 open reading frame 133
• Masse moléculaire calculée: 493 aa, 57 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC015054
• Symbole du gène: VIPAS39
• Identification du gène (NCBI): 63894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; FERARI is required for Rab11-dependent endocytic recycling.; Authors - Jachen A Solinger
human	WB	Virus Genes; Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7.; Authors - Ye Li
human	WB	Adv Sci (Weinh); Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase; Authors - Johannes Naß
human	WB	EBioMedicine; VIPAS39 confers ferroptosis resistance in epithelial ovarian cancer through exporting ACSL4; Authors - Yuening Jiang ; KD Validated
mouse	IF	Elife; Endocytic recycling is central to circadian collagen fibrillogenesis and disrupted in fibrosis; Authors - Joan Chang

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