Anticorps Recombinant de lapin anti-VIPAS39

VIPAS39 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241616A12

N° de cat : 84320-2-PBS

Synonymes

C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39

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Galerie de données de validation

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  WB analysis using 84320-2-RR (same clone as 84320-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84320-2-RR (C14orf133 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84320-2-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84320-2-RR (same clone as 84320-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using VIPAS39 antibody (84320-2-RR, Clone: 241616A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84320-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01194-2

  Cytometric bead array standard curve of MP01194-2, VIPAS39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-3-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01194-3

  Cytometric bead array standard curve of MP01194-3, VIPAS39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-1-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84320-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84320-2-RR against Human VIPAS39 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84320-2-PBS cible VIPAS39 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VIPAS39 Protéine recombinante Ag14762
• Nom complet: chromosome 14 open reading frame 133
• Masse moléculaire calculée: 493 aa, 57 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC015054
• Symbole du gène: VIPAS39
• Identification du gène (NCBI): 63894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

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