Anticorps Polyclonal de lapin anti-C14orf138

C14orf138 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24722-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of PC-3 using 24722-1-AP (same clone as 24722-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 24722-1-AP (same clone as 24722-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 24722-1-AP (same clone as 24722-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24722-1-AP (same clone as 24722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24722-1-AP (same clone as 24722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

24722-1-PBS cible C14orf138 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C14orf138 Protéine recombinante Ag20154
• Nom complet: chromosome 14 open reading frame 138
• Masse moléculaire calculée: 229 aa, 26 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC105118
• Symbole du gène: C14orf138
• Identification du gène (NCBI): 79609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

C14orf138, also named as VCPKMT or METTL21D, is 229 amino acid protein, which belongs to the methyltransferase superfamily. C14orf138 localizes in the cytoplasm and interacts with ALKBH6. C14orf138 as a Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. C14orf138 exists as five isoforms. The calculated molecular weight of C14orf138 is 26 kDa,but we always detected a 46-50 kDa portein by western blot.

{{ptg:RelatedPrimaryAntibodies}}