Anticorps Polyclonal de lapin anti-MYDGF/C19orf10

• Phare
• Validé par KD/KO

MYDGF/C19orf10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 11353-1-AP

Synonymes

C19orf10, MYDGF, Myeloid-derived growth factor, SF20, UPF0556 protein C19orf10

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Galerie de données de validation

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  WB analysis using 11353-1-AP

  various cells were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF/C19orf10F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 11353-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11353-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF/C19orf10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11353-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYDGF/C19orf10 antibody 11353-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 11353-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYDGF/C19orf10 antibody (11353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HeLa, cellules Jurkat, tissu de côlon de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

11353-1-AP cible MYDGF/C19orf10 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYDGF/C19orf10 Protéine recombinante Ag1900
• Nom complet: chromosome 19 open reading frame 10
• Masse moléculaire calculée: 173 aa, 19 kDa
• Poids moléculaire observé: 15-19 kDa
• Numéro d’acquisition GenBank: BC010129
• Symbole du gène: MYDGF/C19orf10
• Identification du gène (NCBI): 56005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).

Publications

Species	Application	Title
mouse	WB,IF	Sci Adv; Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.; Authors - Biying Meng ; KO Validated
Human	WB	Nat Commun; Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis.; Authors - Shu-Yi Yin
rat,mouse,human	WB,IHC,IF	Kidney Int; Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.; Authors - Ping Zhan ; KO Validated
mouse	WB	Front Immunol; Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axis; Authors - Zi Yang
human	WB,IHC	Front Oncol; The Clinical Relevance and Tumor Promoting Function of C19orf10 in Kidney Renal Clear Cell Carcinoma.; Authors - Yanxin Lu ; KD Validated
human	WB,IHC	J Cancer; C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways.; Authors - Shi Li ; KD Validated

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