Anticorps Polyclonal de lapin anti-C1GALT1

C1GALT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 27569-1-AP

Synonymes

B3Gal T8, Beta 1,3 galactosyltransferase, C1GALT, Core 1 beta3 Gal T1, Core 1 O glycan T synthase

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Galerie de données de validation

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  WB analysis of T-47D using 27569-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 27569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27569-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 27569-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1GALT1 antibody (27569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules MKN-45, cellules HepG2, cellules T-47D
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

27569-1-AP cible C1GALT1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C1GALT1 Protéine recombinante Ag26144
• Nom complet: core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: NM_020156
• Symbole du gène: C1GALT1
• Identification du gène (NCBI): 56913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C1GALT1 (Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1), also known as B3Gal-T8, catalyzes the transfer of galactose (Gal) from UDP-Gal to N-acetylgalactosamine (GalNAc) O-linked esters of threonine and serine residues of target proteins, including IgA1, to form the T antigen (PMID: 28209808). C1GalT1 is overexpressed in many common cancers includingcolon, breast, gastric, lung, head and neck, pancreatic, esophageal, prostate, and hepatocellular cancer (PMID: 34944925). C1GALT1 protein contains 363 amino acids and is a typeII transmembrane protein. Rate C1GalT1 is an 84/86KDa disulfide-bonded homodimer, but its active form is believed to be a 42/43 KDa monomeric protein (PMID: 34149900).

Publications

Species	Application	Title
	WB	Int Immunopharmacol; The upregulation of miR-98-5p affects the glycosylation of IgA1 through cytokines in IgA nephropathy.; Authors - Di Liu
human	IHC	J Biomed Res; Genetic variants in C1GALT1 are associated with gastric cancer risk and immune infiltrations; Authors - Mengfan Guo

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