Anticorps Monoclonal anti-C1QA

C1QA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B10E3

N° de cat : 67063-1-PBS

Synonymes

Complement C1q A chain, 1B10E3

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Galerie de données de validation

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  WB analysis of human plasma using 67063-1-Ig (same clone as 67063-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 67063-1-Ig (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

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  WB analysis of human blood using 67063-1-Ig (same clone as 67063-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 67063-1-Ig (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67063-1-Ig (same clone as 67063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67063-1-Ig (C1QA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67063-1-Ig (same clone as 67063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67063-1-Ig (C1QA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67063-1-Ig (same clone as 67063-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (67063-1-Ig, Clone: 1B10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67063-1-PBS cible C1QA dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: C1QA Protéine recombinante Ag28622
• Nom complet: complement component 1, q subcomponent, A chain
• Masse moléculaire calculée: 245 aa, 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC030153
• Symbole du gène: C1QA
• Identification du gène (NCBI): 712
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

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