Anticorps Polyclonal de lapin anti-C1qC

• Phare
• Validé par KD/KO

C1qC Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 16889-1-AP

Synonymes

C1Q C, Complement C1q C chain, C1QG

---

Galerie de données de validation

• 

  WB analysis using 16889-1-AP

  human plasma was subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse serum using 16889-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16889-1-AP

  rat plasma was subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16889-1-AP (C1QC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16889-1-AP (C1QC Antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: plasma humain, sérum de souris
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16889-1-AP cible C1qC dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C1qC Protéine recombinante Ag10400
• Nom complet: complement component 1, q subcomponent, C chain
• Masse moléculaire calculée: 245 aa, 26 kDa
• Poids moléculaire observé: 24-26 kDa
• Numéro d’acquisition GenBank: BC009016
• Symbole du gène: C1QC
• Identification du gène (NCBI): 714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

Publications

Species	Application	Title
human	WB	World J Surg Oncol; Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma; Authors - Wei Dong
human	WB	Front Genet; C1QC is a prognostic biomarker with immune-related value in kidney renal clear cell carcinoma; Authors - Wentao Yao ; KD Validated
human	IHC	J Cancer; Identification of crucial genes through WGCNA in the progression of gastric cancer; Authors - Rui Liu
mouse	WB,IF	Mol Neurobiol; Upregulation of C1QC as a Mediator of Blood-Brain Barrier Damage in Type 2 Diabetes Mellitus; Authors - Cheng Huang
human	IHC	Sci Rep; Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies; Authors - Yang Yang

{{ptg:RelatedPrimaryAntibodies}}