Anticorps Recombinant de lapin anti-C1qC

C1qC Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242624B1

N° de cat : 85031-5-PBS

Synonymes

Complement C1q C chain, C1Q C, 242624B1, C1QG

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Galerie de données de validation

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  WB analysis of human plasma using 85031-5-RR (same clone as 85031-5-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 85031-5-RR (C1QC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85031-5-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 85031-5-RR (same clone as 85031-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85031-5-RR (C1qC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85031-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85031-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85031-5-RR against Human C1qC were performed. The affinity constant is 1.26 nM.

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Informations sur le produit

85031-5-PBS cible C1qC dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: C1qC Protéine recombinante Ag10400
• Nom complet: complement component 1, q subcomponent, C chain
• Masse moléculaire calculée: 245 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC009016
• Symbole du gène: C1QC
• Identification du gène (NCBI): 714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

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