Anticorps Polyclonal de lapin anti-COA6

• Phare
• Validé par KD/KO

COA6 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 24209-1-AP

Synonymes

C1orf31

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Galerie de données de validation

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  WB analysis of HEK-293 using 24209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24209-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 24209-1-AP

  IP result of anti-COA6 (IP:24209-1-AP, 4ug; Detection:24209-1-AP 1:500) with HEK-293 cells lysate 1760ug.

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  IHC staining of human kidney using 24209-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24209-1-AP (COA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24209-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24209-1-AP (COA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 24209-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf31 antibody (24209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

24209-1-AP cible COA6 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COA6 Protéine recombinante Ag21525
• Nom complet: chromosome 1 open reading frame 31
• Masse moléculaire calculée: 125 aa, 14 kDa
• Poids moléculaire observé: 10 - 18 kDa
• Numéro d’acquisition GenBank: BC116455
• Symbole du gène: COA6
• Identification du gène (NCBI): 388753
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C1orf31, also known as COA6, is a conserved mitochondrial protein required for respiratory complex IV biogenesis. Recently study reported that C1orf31 is required for maintenance of cytochrome c oxidase (CcO) subunit levels, and has role in the Cu delivery pathway to CcO. Defects in C1orf31 is associated with mitochondrial respiratory chain disease (MRCD). (24549041)

Publications

Species	Application	Title
human	WB	Hum Mol Genet; Copper supplementation restores cytochrome c oxidase assembly defect in a mitochondrial disease model of COA6 deficiency.; Authors - Alok Ghosh ; KD Validated
human	WB	Cell Rep; COA6 Is Structurally Tuned to Function as a Thiol-Disulfide Oxidoreductase in Copper Delivery to Mitochondrial Cytochrome c Oxidase.; Authors - Shivatheja Soma
human	WB,IF	Int J Mol Sci; High Expression of COA6 Is Related to Unfavorable Prognosis and Enhanced Oxidative Phosphorylation in Lung Adenocarcinoma; Authors - Ming Zhang ; KD Validated
human	IHC	Heliyon; Prognostic value and immunological function of cuproptosis-related genes in lung adenocarcinoma; Authors - Liming Zhang
human	WB	Sci Rep; Oxidative phosphorylation related gene COA6 is a novel indicator for the prognosis and immune response in lung adenocarcinoma; Authors - Wenting Liu

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