Anticorps Recombinant de lapin anti-C1orf77

C1orf77 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242770H3

N° de cat : 85147-3-PBS

Synonymes

CHTOP, FOP, 242770H3, Friend of PRMT1 protein, PP7704

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Galerie de données de validation

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  WB analysis using 85147-3-RR (same clone as 85147-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85147-3-RR (C1orf77 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85147-3-RR (same clone as 85147-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85147-3-RR (same clone as 85147-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 85147-3-RR (same clone as 85147-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85147-3-RR (same clone as 85147-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 85147-3-RR (same clone as 85147-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 85147-3-RR (same clone as 85147-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01863-1

  Cytometric bead array standard curve of MP01863-1, C1orf77 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85147-3-PBS. Detection antibody: 85147-1-PBS. Standard: Ag26580. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 85147-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85147-3-RR against Human C1orf77 were performed. The affinity constant is 9.63 nM.

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Informations sur le produit

85147-3-PBS cible C1orf77 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: C1orf77 Protéine recombinante Ag26580
• Nom complet: chromosome 1 open reading frame 77
• Masse moléculaire calculée: 248 aa, 26 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC002733
• Symbole du gène: C1orf77
• Identification du gène (NCBI): 26097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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