Anticorps Polyclonal de lapin anti-C1orf83

C1orf83 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 20940-1-AP

Synonymes

FLJ32112, FLJ39169, RP4 758J24.3, TCEANC2, Transcription elongation factor A N-terminal and central domain-containing protein 2

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Galerie de données de validation

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  WB analysis using 20940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20940-1-AP (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 20940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf83 antibody (20940-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris, tissu cérébral humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20940-1-AP cible C1orf83 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C1orf83 Protéine recombinante Ag15040
• Nom complet: chromosome 1 open reading frame 83
• Masse moléculaire calculée: 208 aa, 23 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC035374
• Symbole du gène: C1orf83
• Identification du gène (NCBI): 127428
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).

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