Anticorps Recombinant de lapin anti-C1orf83

C1orf83 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240678D10

N° de cat : 83626-3-PBS

Synonymes

240678D10, FLJ32112, FLJ39169, RP4 758J24.3, TCEANC2

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Galerie de données de validation

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  IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83626-3-RR (same clone as 83626-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83626-3-RR (same clone as 83626-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00578-2

  Cytometric bead array standard curve of MP00578-2, C1orf83 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83626-1-PBS. Detection antibody: 83626-3-PBS. Standard: Ag15040. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83626-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83626-3-PBS cible C1orf83 dans les applications de IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: C1orf83 Protéine recombinante Ag15040
• Nom complet: chromosome 1 open reading frame 83
• Masse moléculaire calculée: 208 aa, 23 kDa
• Numéro d’acquisition GenBank: BC035374
• Symbole du gène: C1orf83
• Identification du gène (NCBI): 127428
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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