Anticorps Recombinant de lapin anti-C1r

C1r Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242596F5

N° de cat : 85029-4-PBS

Synonymes

242596F5, Complement C1r, Complement C1r subcomponent, Complement C1r subcomponent heavy chain, Complement C1r subcomponent light chain

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Galerie de données de validation

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  WB analysis of human plasma using 85029-4-RR (same clone as 85029-4-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 85029-4-RR (C1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85029-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85029-4-RR (same clone as 85029-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85029-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85029-4-RR (same clone as 85029-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85029-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85029-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85029-4-RR against Human C1r were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85029-4-PBS cible C1r dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: C1r Protéine recombinante Ag11390
• Nom complet: complement component 1, r subcomponent
• Masse moléculaire calculée: 705 aa, 80 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC035220
• Symbole du gène: C1R
• Identification du gène (NCBI): 715
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein that can be cleaved into an A chain and a B chain upon activation.

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