Anticorps Polyclonal de lapin anti-GID8

GID8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 24479-1-AP

Synonymes

C20orf11, Glucose-induced degradation protein 8 homolog, Protein C20orf11, Twa1, Two hybrid-associated protein 1 with RanBPM



Applications testées

Résultats positifs en WBcellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules SW480, tissu cérébral de rat, tissu cérébral de souris
Résultats positifs en IHCtissu testiculaire de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

24479-1-AP cible GID8 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène GID8 Protéine recombinante Ag19751
Nom complet chromosome 20 open reading frame 11
Masse moléculaire calculée 228 aa, 27 kDa
Poids moléculaire observé 30 kDa
Numéro d’acquisition GenBankBC032120
Symbole du gène GID8
Identification du gène (NCBI) 54994
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The GID8 protein is a component of the Glucose-Induced Degradation-deficient (GID) complex, which plays a key role in ubiquitin-mediated protein degradation, a pathway used to regulate protein turnover in eukaryotic cells. Initially discovered in yeast, GID8 acts as a scaffold protein that is essential for the assembly of the multi-subunit GID complex and for substrate recognition. This complex plays a significant role in metabolic regulation, such as degrading fructose-1,6-bisphosphatase (FBPase) during the shift from gluconeogenesis to glycolysis. In humans, GID8 is part of the CTLH complex, which is associated with various cellular processes including cell cycle regulation, apoptosis, and cancer progression.

Protocole

Product Specific Protocols
WB protocol for GID8 antibody 24479-1-APDownload protocol
IHC protocol for GID8 antibody 24479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex.

Authors - Lucia Carolina Leal-Esteban
humanWB

Sci Rep

Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.

Authors - Shimaa H A Soliman
humanWB

bioRxiv

Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth

Authors - Nelson V Simwela
humanWB,IHC,IF

Cancer Lett

YTHDF1 regulates GID8-mediated glutamine metabolism to promote colorectal cancer progression in a m6A-dependent manner

Authors - Yicun Han
humanWB

Mol Cell

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Authors - Sang Ah Yi
mouseWB

Nat Commun

Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth

Authors - Nelson V Simwela

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

H (Verified Customer) (09-27-2020)

GID8 was pulled down with MAEA-flag, so I could tell which band is GID8. But without IP, it would be difficult to tell which band is the GID8, because the on-target band signal is too weak, while off-target band signals are strong in HEK293T cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
GID8 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:24479-1-AP)
{{ptg:RelatedPrimaryAntibodies}}