Anticorps Polyclonal de lapin anti-GID8

GID8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 24479-1-AP

Synonymes

C20orf11, GID8, Protein C20orf11, TWA1

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Galerie de données de validation

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  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24479-1-AP (GID8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24479-1-AP (GID8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules SW480, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

24479-1-AP cible GID8 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GID8 Protéine recombinante Ag19751
• Nom complet: chromosome 20 open reading frame 11
• Masse moléculaire calculée: 228 aa, 27 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC032120
• Symbole du gène: GID8
• Identification du gène (NCBI): 54994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	PLoS Genet; Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex.; Authors - Lucia Carolina Leal-Esteban
human	WB	Sci Rep; Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.; Authors - Shimaa H A Soliman

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (09-27-2020)

GID8 was pulled down with MAEA-flag, so I could tell which band is GID8. But without IP, it would be difficult to tell which band is the GID8, because the on-target band signal is too weak, while off-target band signals are strong in HEK293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

GID8 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:24479-1-AP)