Anticorps Polyclonal de lapin anti-C20orf20

• Phare
• Validé par KD/KO

C20orf20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26040-1-PBS

Synonymes

Eaf7, MRG/MORF4L-binding protein, MRG15BP, MRG-binding protein, MRGBP

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Galerie de données de validation

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  WB analysis of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 26040-1-AP (same clone as 26040-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

  IP result of anti-C20orf20 (IP:26040-1-AP, 4ug; Detection:26040-1-AP 1:300) with HepG2 cells lysate 2640ug. This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 26040-1-AP (same clone as 26040-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 26040-1-AP (same clone as 26040-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C20orf20 antibody (26040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26040-1-PBS cible C20orf20 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C20orf20 Protéine recombinante Ag22840
• Nom complet: chromosome 20 open reading frame 20
• Masse moléculaire calculée: 204 aa, 22 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC014235
• Symbole du gène: C20orf20
• Identification du gène (NCBI): 55257
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

C20orf20, also named as MRG/MORF4L-binding protein or Up-regulated in colon cancer 4 (Urcc4), is a 204 amino acid protein, which belongs to the EAF7 family. C20orf20 localizes in the nucleus and is a Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. C20orf20 is modified through phosphorylation and acetylation, so the molecular weight of C20orf20 might be 25-30 kDa.

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