Anticorps Recombinant de lapin anti-RTCB

RTCB Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250866B2

N° de cat : 86258-4-PBS

Synonymes

HSPC117, 3'-phosphate/5'-hydroxy nucleic acid ligase, C22orf28, EC:6.5.1.8, FAAP

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Galerie de données de validation

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  WB analysis using 86258-4-RR (same clone as 86258-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86258-4-RR (C22orf28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86258-4-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 86258-4-RR (same clone as 86258-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RTCB antibody (86258-4-RR, Clone: 250866B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86258-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86258-4-PBS

  Biolayer interferometry (BLl) kinetic assays of 86258-4-PBS against Human RTCB were performed. The affinity constant is 0.303 nM.

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Informations sur le produit

86258-4-PBS cible RTCB dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RTCB Protéine recombinante Ag13836
• Nom complet: chromosome 22 open reading frame 28
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000151
• Symbole du gène: RTCB
• Identification du gène (NCBI): 51493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

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