Anticorps Polyclonal de lapin anti-C4 Gamma Chain

C4 Gamma Chain Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17160-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human plasma using 17160-1-AP (same clone as 17160-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 17160-1-AP (same clone as 17160-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 17160-1-AP (same clone as 17160-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17160-1-PBS cible C4 Gamma Chain dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C4 Gamma Chain Protéine recombinante Ag10449
• Nom complet: complement component 4A (Rodgers blood group)
• Masse moléculaire calculée: 1744 aa, 193 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC012372
• Symbole du gène: C4 Gamma Chain
• Identification du gène (NCBI): 720
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4A protein recognizes the C4 gamma chain.

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