Anticorps Recombinant de lapin anti-C5AR1/CD88

C5AR1/CD88 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241712G8

N° de cat : 84336-6-PBS

Synonymes

C5AR, C5AR1, 241712G8, C5A, C5a anaphylatoxin chemotactic receptor 1

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Galerie de données de validation

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  WB analysis using 84336-6-RR (same clone as 84336-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84336-6-RR (C5AR1/CD88 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84336-6-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 84336-6-RR (same clone as 84336-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using C5AR1/CD88 antibody (84336-6-RR, Clone: 241712G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84336-6-PBS in a different storage buffer formulation.

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Informations sur le produit

84336-6-PBS cible C5AR1/CD88 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: C5AR1/CD88 Protéine recombinante Eg2185
• Nom complet: complement component 5a receptor 1
• Masse moléculaire calculée: 39kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_001736.4
• Symbole du gène: C5aR
• Identification du gène (NCBI): 728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).

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