Anticorps Polyclonal de lapin anti-C6orf134

• Phare
• Validé par KD/KO

C6orf134 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 28828-1-AP

Synonymes

MEC17, Alpha-TAT, ATAT1, C6orf134, DKFZp547J097, FLJ13158, Nbla00487, TAT, UPF0635 protein C6orf134

---

Galerie de données de validation

• 

  WB analysis using 28828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (C6orf134 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28828-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (C6orf134 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat testis using 28828-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28828-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 28828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using C6orf134 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 28828-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C6orf134 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 28828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using C6orf134 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 28828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using C6orf134 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of A431 using 28828-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human C6orf134 (28828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules MG U-87, cellules THP-1, cellules U-251
• Résultats positifs en IHC: tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

28828-1-AP cible C6orf134 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C6orf134 Protéine recombinante Ag28757
• Nom complet: chromosome 6 open reading frame 134
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: NM_001031722
• Symbole du gène: C6orf134
• Identification du gène (NCBI): 79969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.

Publications

Species	Application	Title
human	WB	MedComm (2020); Matrix stiffness-induced α-tubulin acetylation is required for skin fibrosis formation through activation of Yes-associated protein; Authors - Dongsheng Wen ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

K. (Verified Customer) (10-26-2023)

Each well had 20 ug of protein extract added. On a Nitrocellulose Blotting Membrane, transfer was carried out for two hours at 400 mA at 4 oC. Membrane blocking was carried out at room temperature with 5% BSA in TBS-Tween20 under light shaking. Antibodies were diluted in PBS-Tween20 containing 5% non-fat milk. -The primary antibody incubations were carried out as follows: 1:1000 for three hours at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human and mouse Liver cells