Anticorps Polyclonal de lapin anti-C9orf72

• Phare
• Validé par KD/KO

C9orf72 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(36)

N° de cat : 22637-1-AP

Synonymes

C9RANT, DENND9, DENNL72, Guanine nucleotide exchange factor C9orf72

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Galerie de données de validation

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  WB analysis of rat brain using 22637-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22637-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22637-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 22637-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22637-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 22637-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of transfected cells using 22637-1-AP

  WB result of anti-C9ORF72 (22637-1-AP) with Variant1 and 2 transfected. (by Dr. Sarah Mueller).

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  IP experiment of SH-SY5Y using 22637-1-AP

  IP result of anti-C9orf72 (IP:22637-1-AP, 3ug; Detection:22637-1-AP 1:300) with SH-SY5Y cells lysate 2000ug.

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  IHC staining of human gliomas using 22637-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22637-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 22637-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22637-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22637-1-AP (C9orf72 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22637-1-AP (C9orf72 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 22637-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22637-1-AP (C9orf72 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22637-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22637-1-AP (C9orf72 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 22637-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22637-1-AP (C9orf72 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human gliomas using 22637-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22637-1-AP (C9orf72 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 22637-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9orf72 antibody (22637-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules C2C12, cellules HEK-293, cellules HeLa, cellules NIH/3T3, cellules SH-SY5Y, cellules Sp2/0, cellules transfectées, tissu cérébral de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 17 publications below
• WB: See 34 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• CoIP: See 2 publications below

Informations sur le produit

22637-1-AP cible C9orf72 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Caenorhabditis elegans
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C9orf72 Protéine recombinante Ag18326
• Nom complet: chromosome 9 open reading frame 72
• Masse moléculaire calculée: 481 aa, 54 kDa
• Poids moléculaire observé: 50-54 kDa
• Numéro d’acquisition GenBank: BC020851
• Symbole du gène: C9orf72
• Identification du gène (NCBI): 203228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. It has been reported that C9orf72 forms a complex with Cofilin and other actin binding proteins and 22637-1-AP antibody detects the complex bands around 43 and 68-72 kDa in SDS-PAGE.(PMID: 27723745)

Publications

Species	Application	Title
mouse	WB	Science; C9orf72 is required for proper macrophage and microglial function in mice.; Authors - J G O'Rourke ; KO Validated
human	wb	Nat Med; Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.; Authors - Yingxiao Shi
mouse,human	WB,CoIP	Cell Metab; C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.; Authors - Tao Wang ; KO Validated
mouse,human	WB	Nat Neurosci; Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.; Authors - Qiang Zhu ; KO Validated
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang ; KO Validated
mouse	WB	Sci Transl Med; Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.; Authors - Aaron Burberry ; KO Validated

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