Anticorps Monoclonal anti-C9orf72

C9orf72 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

3D2H6

N° de cat : 66140-1-Ig

Synonymes

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 3D2H6

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Galerie de données de validation

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  WB analysis of human brain using 66140-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 66140-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66140-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of C6 using 66140-1-Ig

  IP result of anti-C9orf72 (IP:66140-1-Ig, 4ug; Detection:66140-1-Ig 1:1000) with C6 cells lysate 1320ug.

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  IHC staining of human gliomas using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 66140-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9orf72 antibody (66140-1-Ig, Clone: 3D2H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules C6, cellules Neuro-2a
• Résultats positifs en IP: cellules C6
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66140-1-Ig cible C9orf72 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: C9orf72 Protéine recombinante Ag21080
• Nom complet: chromosome 9 open reading frame 72
• Masse moléculaire calculée: 481 aa, 54 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC020851
• Symbole du gène: C9orf72
• Identification du gène (NCBI): 203228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.

Publications

Species	Application	Title
human,mouse	WB,IF	Acta Neuropathol Commun; Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.; Authors - Petra Frick
human	WB	Sci Rep; Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo.; Authors - Xuejiao Piao
human	IHC	Amyotroph Lateral Scler Frontotemporal Degener; Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.; Authors - Yvonne S Davidson
human	IHC	Acta Neuropathol; Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.; Authors - Shahram Saberi
rat	WB	Animal Model Exp Med; Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats.; Authors - Wei Dong
human	WB	Nat Neurosci; C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.; Authors - Rajeeve Sivadasan

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