Anticorps Polyclonal de lapin anti-SPACA9

SPACA9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 26034-1-AP

Synonymes

C9orf9, Sperm acrosome-associated protein 9, Uncharacterized protein C9orf9

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Galerie de données de validation

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  WB analysis of mouse testis using 26034-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 26034-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26034-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 26034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26034-1-AP (SPACA9 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 26034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26034-1-AP (SPACA9 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 26034-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SPACA9 antibody (26034-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat testis using 26034-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using SPACA9 antibody (26034-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 26034-1-AP

  Immunofluorescent analysis of A431 cells using 26034-1-AP (C9orf9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HaCaT using 26034-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using SPACA9 antibody (26034-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 26034-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPACA9 antibody (26034-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu hépatique de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431, cellules HaCaT

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

26034-1-AP cible SPACA9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPACA9 Protéine recombinante Ag23320
• Nom complet: chromosome 9 open reading frame 9
• Poids moléculaire observé: 18 kDa, 30 kDa
• Numéro d’acquisition GenBank: BC012940
• Symbole du gène: SPACA9
• Identification du gène (NCBI): 11092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The function of C9orf9 remains largely unknown. Catalog#26034-1-AP is a rabbit polyclonal antibody raised against the full-length of human C9orf9. Two isoforms were produced by alternative splicing with predicted MW of 19 and 26 kDa (UniProt). Two bands at 16 kDa and 30 kDa could be detected using the present rabbit polyclonal antibody 26034-1-AP.

Publications

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma

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