Anticorps Polyclonal de lapin anti-CA1

CA1 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 13198-2-AP

Synonymes

CA I, CA1, CAB, Car1, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, carbonic anhydrase I

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Galerie de données de validation

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  WB analysis using 13198-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13198-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 13198-2-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13198-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13198-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13198-2-AP

  IP Result of anti-CA1 (IP:13198-2-AP, 4ug; Detection:13198-2-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human breast cancer using 13198-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13198-2-AP (CA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13198-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13198-2-AP (CA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules Jurkat, tissu splénique de rat
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

13198-2-AP cible CA1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CA1 Protéine recombinante Ag3932
• Nom complet: carbonic anhydrase I
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC027890
• Symbole du gène: CA1
• Identification du gène (NCBI): 759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CA1(Carbonic anhydrase 1) is also named as CAB and belongs to the alpha-carbonic anhydrase family, which may reside in cytoplasm, in mitochondria, or in secretory granules, or associate with membranes in cell. It forms a large family of genes encoding zinc metalloenzymes of great physiologic importance. Extracellular CA1 mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation(PMID:17259996).

Publications

Species	Application	Title
mouse,rat	WB,IF	Acta Neuropathol Commun; Upregulation of carbonic anhydrase 1 beneficial for depressive disorder; Authors - Ke Cheng ; KO Validated
rat	WB	J Ginseng Res; Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway; Authors - Mei-Li Lu
mouse	IF	FASEB J; Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.; Authors - Brittany R Jenkins
human	WB	J Inflamm Res; M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs; Authors - Xianqin Song
mouse	WB	Am J Physiol Lung Cell Mol Physiol; Carbonic anhydrase and soluble adenylate cyclase regulation of cystic fibrosis cellular phenotypes.; Authors - Kathleen Boyne
human,rat	WB	Front Pharmacol; Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.; Authors - Meili Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brittany (Verified Customer) (09-27-2019)

Using the CA-1 primary antibody with mouse colon scrapes yielded very clean bands with western blotting. Immunofluorescence also gave a fairly clean, detectable signal, with CA-1 showing up along the brush border in mouse colon tissue. There were some cells that stained more intracellularly, and unsure if that was non-specific binding of the antibody, or another cell type other than colonocytes that expresses CA-1 intracellularly.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 (WB) and 1:100 (IF)
• Cell Tissue Type: Colon tissue (scrapes for western blot)

CA1 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB) and 1:100 (IF) dilution) in Colon tissue (scrapes for western blot) (Cat no:13198-2-AP)