Anticorps Recombinant de lapin anti-CA11

CA11 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230390F7

N° de cat : 83082-4-PBS

Synonymes

230390F7, CA RP II, CA RP XI, CA XI, carbonic anhydrase XI

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Galerie de données de validation

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  IF Staining of U2OS using 83082-4-RR (same clone as 83082-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CA11 antibody (83082-4-RR, Clone: 230390F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83082-4-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83082-4-RR (same clone as 83082-4-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug CA11 Recombinant antibody (83082-4-RR, Clone:230390F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83082-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83082-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83042-4-RR against Human CA11 were performed. The affinity constant is 0.78 nM.

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Informations sur le produit

83082-4-PBS cible CA11 dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CA11 Protéine recombinante Ag33373
• Nom complet: carbonic anhydrase XI
• Masse moléculaire calculée: 36 kDa
• Numéro d’acquisition GenBank: BC002662
• Symbole du gène: CA11
• Identification du gène (NCBI): 770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CA11(Carbonic anhydrase-related protein 11) is also named CARP2, and CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical, and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).

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