Anticorps Monoclonal anti-CA12

CA12 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E1D8

N° de cat : 68224-1-PBS

Synonymes

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

---

Galerie de données de validation

• 

  WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 68224-1-Ig (same clone as 68224-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody (68224-1-Ig, Clone: 3E1D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68224-1-PBS cible CA12 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CA12 Protéine recombinante Ag7235
• Nom complet: carbonic anhydrase XII
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 38-45 kDa
• Numéro d’acquisition GenBank: BC000278
• Symbole du gène: CA12
• Identification du gène (NCBI): 771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

{{ptg:RelatedPrimaryAntibodies}}