Anticorps Recombinant de lapin anti-CA12

CA12 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

243053B2

N° de cat : 85595-3-PBS

Synonymes

CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII

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Galerie de données de validation

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  WB analysis using 85595-3-RR (same clone as 85595-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85595-3-RR (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85595-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01993-1

  Sandwich ELISA standard curve of MP01993-1, Human CA12 Recombinant Matched Antibody Pair - PBS only. 85595-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7107. 85595-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  Sample test of MP01993-1

  The mean CA12 concentration was determined to be 93.99 ng/mL in A549 cell extract based on a 3.10 mg/mL extract load.

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  Cytometric bead array standard curve of MP01993-1

  Cytometric bead array standard curve of MP01993-1, CA12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85595-3-PBS. Detection antibody: 85595-2-PBS. Standard: Ag7107. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85595-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85595-3-RR against Human CA12 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85595-3-PBS cible CA12 dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CA12 Protéine recombinante Ag7107
• Nom complet: carbonic anhydrase XII
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 38-45 kDa
• Numéro d’acquisition GenBank: BC000278
• Symbole du gène: CA12
• Identification du gène (NCBI): 771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

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