Anticorps Recombinant de lapin anti-CA13

CA13 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250787A12

N° de cat : 86178-1-RR

Synonymes

CA XIII, Carbonate dehydratase XIII, Carbonic anhydrase 13, Carbonic anhydrase XIII, CAXIII

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 86178-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86178-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 86178-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86178-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86178-1-RR against Human CA13 were performed. The affinity constant is 4.50 nM.

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NIH/3T3, tissu de thymus de souris, tissu d'intestin grêle de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86178-1-RR cible CA13 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CA13 Protéine recombinante Ag10086
• Nom complet: carbonic anhydrase XIII
• Masse moléculaire calculée: 262 aa, 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC052602
• Symbole du gène: CA13
• Identification du gène (NCBI): 377677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze the reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic protein that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).

{{ptg:RelatedPrimaryAntibodies}}