Anticorps Polyclonal de lapin anti-CA2

CA2 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16961-1-PBS

Synonymes

Carbonate dehydratase II, Car2, CA-II, CAII, CAC

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Galerie de données de validation

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  WB analysis using 16961-1-AP (same clone as 16961-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 16961-1-AP (same clone as 16961-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of human stomach using 16961-1-AP (same clone as 16961-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 16961-1-AP (same clone as 16961-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 16961-1-AP (same clone as 16961-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 16961-1-AP (same clone as 16961-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

  IP result of anti-CA2 (IP:16961-1-AP, 4ug; Detection:16961-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16961-1-PBS cible CA2 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CA2 Protéine recombinante Ag10680
• Nom complet: carbonic anhydrase II
• Masse moléculaire calculée: 260 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC011949
• Symbole du gène: CA2
• Identification du gène (NCBI): 760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).

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