Anticorps Recombinant de lapin anti-CA8

CA8 Recombinant Antibody for IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250429B8

N° de cat : 86004-1-PBS

Synonymes

carbonic anhydrase VIII, CA 8, CA VIII, CALS, Carbonic anhydrase-related protein

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Galerie de données de validation

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  IHC staining of Mouse cerebellum using 86004-1-RR (same clone as 86004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue slide using 86004-1-RR (CA8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86004-1-PBS in a different storage buffer formulation.

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  IHC staining of Mouse cerebellum using 86004-1-RR (same clone as 86004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue slide using 86004-1-RR (CA8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86004-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86004-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86004-1-RR against Human CA8 were performed. The affinity constant is 0.106 nM.

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Informations sur le produit

86004-1-PBS cible CA8 dans les applications de IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CA8 Protéine recombinante Ag3068
• Nom complet: carbonic anhydrase VIII
• Masse moléculaire calculée: 290 aa, 32 kDa
• Numéro d’acquisition GenBank: BC015531
• Symbole du gène: CA8
• Identification du gène (NCBI): 767
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874).

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