Anticorps Recombinant de lapin anti-CA8

CA8 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250429C4

N° de cat : 86004-2-RR

Synonymes

carbonic anhydrase VIII, CA 8, CA VIII, CALS, Carbonic anhydrase-related protein

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Galerie de données de validation

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  WB analysis using 86004-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86004-2-RR (CA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86004-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86004-2-RR (CA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86004-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86004-2-RR against Human CA8 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules K-562, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86004-2-RR cible CA8 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CA8 Protéine recombinante Ag3068
• Nom complet: carbonic anhydrase VIII
• Masse moléculaire calculée: 290 aa, 32 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: BC015531
• Symbole du gène: CA8
• Identification du gène (NCBI): 767
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874).

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